Abstract:

To investigate the role of RBP4 during the differentiation of porcine preadipocytes and the biological function of RBP4, we constructed porcine RBP4 lentivirus interfering vector, then infected the porcine preadipocytes. The changes in morphology and gene expression levels were studied to evaluate the effects of RBP4 expression on preadipocyte differentiation by BODIPY, Oil Red O staining and real-time PCR analysis. The results showed that RBP4 lentivirus interfering vector was constructed correctly, virus titer was 6.5×10⁷ pfu·mL^-1. RBP4 mRNA and protein expression was remarkably reduced by approximate 60% in porcine preadipocytes infected with shRBP4. Moreover, preadipocytes differentiation was promoted and the mRNA expression levels of PPARγ, SREBP-1c and aP2 were increased in adipocytes by knocking down of RBP4. Therefore, adipogenic differentiation of porcine preadipocytes are inhibited by RBP4, which would help the further exploration of the biological mechanism of RBP4 during the adipogenesis.